![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K2926088:Design
Designed by: Johanna Opgenoorth Group: iGEM19_Bielefeld-CeBiTec (2019-10-21)
Basic Insert to express Cas13a (Lsh) and a short guideRNA array in S. cerevisiae
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 5227
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 5252
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1016
Illegal BglII site found at 1562
Illegal BglII site found at 1640
Illegal BglII site found at 2327
Illegal BglII site found at 3215
Illegal BglII site found at 4205 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 5227
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 5227
Illegal AgeI site found at 55 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 783
Illegal SapI.rc site found at 1108
Illegal SapI.rc site found at 1978
Illegal SapI.rc site found at 2812
Design Notes
Due to the repetitivity of the guide RNA cluster we assembled it using Golden Gate Assembly.
Source
Promoters and terminators were obtained via gene synthesis while Cas13a was excised from Munich 2017s plasmid and the guide RNA cluster was cloned via Golden Gate Assembly.